30th anniversary of M Grenson’s paper reporting the first isolation of rsp5 mutants of yeast

In 1983, Marcelle Grenson published her famous paper describing the “inactivation” by ammonium of the yeast Gap1 permease, and the isolation of a mut2 mutant in which this inactivation is defective [1]. Later studies [2] showed that the mut2 mutation (also named npi1) alters the RSP5 gene encoding the HECT-type ubiquitin ligase enzyme [3] responsible for the ubiquitylation of all thus far reported plasma membrane proteins of yeast, including Gap1 [4].

Grenson (1983) – Inactivation-Reactivation Process and Repression of Permease Formation Regulate Several Ammonia-Sensitive Permeases in the Yeast Saccharomyces cerevisiae. Eur J Biochem 133, 135-139.

Marcelle Grenson